The polo-like kinase 1 regulates CDC25B-dependent mitosis entry

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Polo-like kinase 1 regulates the stability of the mitotic centromere-associated kinesin in mitosis

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Whether mitotic structures like the centrosome can self-organize from the regulated mobility of their dynamic protein components remains unclear. Here, we combine fluorescence spectroscopy and chemical genetics to study in living cells the diffusion of polo-like kinase 1 (PLK1), an enzyme critical for centrosome maturation at the onset of mitosis. The cytoplasmic diffusion of a functional EGFP-...

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The Catalytic Subunit of DNA-Dependent Protein Kinase Coordinates with Polo-Like Kinase 1 to Facilitate Mitotic Entry.

DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is the key regulator of the non-homologous end joining pathway of DNA double-strand break repair. We have previously reported that DNA-PKcs is required for maintaining chromosomal stability and mitosis progression. Our further investigations reveal that deficiency in DNA-PKcs activity caused a delay in mitotic entry due to dysregulation ...

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ژورنال

عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

سال: 2009

ISSN: 0167-4889

DOI: 10.1016/j.bbamcr.2008.12.015